Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: The Wave bioreactor creates a more stable culture environment for rapidly expanding lymphocytes . A comparison of media depletion for a culture grown in static bags (circles) or the WAVE bioreactor (triangles). a) glucose concentration. b) lactate concentration. c) pH.

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques: Comparison, Concentration Assay

Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: The WAVE bioreactor and static bags expand T lymphocytes comparably . A comparison of TIL expanded in either static bags (n = 25, circles) or the WAVE bioreactor (n = 27, triangles). a) Total TIL number expanded b) Fold expansion.

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques: Comparison

Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: The WAVE bioreactor and static bags expand the same lymphocyte cultures comparably . a) A comparison of the fold expansion of TIL that underwent concurrent rapid expansion in static bags (circles) and the WAVE bioreactor (triangles). b) A comparison of the fold expansion of genetically modified PBL that underwent concurrent rapid expansion in static bags (circles) and the WAVE bioreactor (triangles).

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques: Comparison, Genetically Modified

Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: A table of IFN-γ secretion in response to HLA matched and mismatched cell lines and to T2 cell pulsed with peptide recognized by the transduced TCR

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques:

Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: The WAVE bioreactor produces cultures with greater numbers of CD4+ cells than static bags . The cell surface expression profile of TIL a) CD3 b) CD4 c) CD8 and genetically modified PBL d) CD3 e) CD4 f) CD8 g) CD4 and Tetramer h) CD8 Tetramer in static bags (circles) and the WAVE bioreactor (triangles).

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques: Expressing, Genetically Modified

Journal: Journal of Translational Medicine

Article Title: Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

doi: 10.1186/1479-5876-10-69

Figure Lengend Snippet: The WAVE bioreactor and static bags produce cultures with a different phenotypic composition . A comparison of TIL activation status by cell surface expression of a) CD8 and CD28 b) CD8 and CD62L and genetically modified PBL activation status by cell surface expression of c) CD4 CD27 d) CD8 CD27 in static bags (circles) and the WAVE bioreactor (triangles).

Article Snippet: The expression of the following cell surface markers on TIL and genetically modified PBL expanded in LifeCell bags and the WAVE bioreactor were compared by 6 color flow cytometry on a Canto II Flow cytometer: CD3, CD4, CD8, CD56, CD45RA, CD45RO, CD62L, CCR7, CD25, CD27, CD28 (BD Biosciences), In addition, for the genetically modified PBL, the expression of the introduced TCR was analyzed by staining with the cognate tetramer complex (Beckman).

Techniques: Comparison, Activation Assay, Expressing, Genetically Modified

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Patterns of viable cell density and viability corresponding to Sf9 cells in SF900 III culture medium in Schott flask and bioreactor. ( A ) Viable cell density pattern. ( B ) Viability pattern. The points and error bars from the Schott flask assays represent the average and the standard deviation of three repetitions, respectively.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Standard Deviation

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Patterns of viable cell density and viability corresponding to Sf9 cells infected with recombinant baculovirus carrying the rabies G glycoprotein at 0.1 pfu/cell MOI in Schott flask and bioreactor. ( A ) Viable cell density pattern. ( B ) Viability pattern. The points and error bars from Schott flask assays represent the average and the standard deviation of three repetitions, respectively.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Infection, Recombinant, Standard Deviation

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Patterns of viable cell density and viability corresponding to Sf9 cells infected with recombinant baculovirus carrying the rabies M protein at 0.1 pfu/cell MOI in Schott flask and bioreactor. ( A ) Viable cell density pattern. ( B ) Viability pattern. The points and error bars from Schott flask assays represent the average and the standard deviation of three repetitions, respectively.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Infection, Recombinant, Standard Deviation

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Kinetics of recombinant baculovirus production (pfu/cell) in Schott flask and bioreactor. ( A ) Kinetic production patterns of recombinant baculovirus carrying the rabies G glycoprotein. ( B ) Kinetic production patterns of recombinant baculovirus carrying the rabies M protein.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Recombinant

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Predicted values of protein concentrations in Schott flasks for optimal conditions and for those that were implemented in bench bioreactor (BVM MOI of 2.0 pfu/cell, 1.5 BVG/BVM MOI ratio), and corresponding empirical values in the last conditions using stirred-tank bioreactor as culture system. Columns represent predicted values by statistical models or the average from three experimental repetitions. Error bars depict the standard deviation from triplicate empirical runs. The letters represent statistically similar values for each protein concentration quantification technique.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Standard Deviation, Protein Concentration

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Patterns of viable cell density and viability corresponding to Sf9 cells coinfected with recombinant baculovirus carrying the rabies G glycoprotein and M protein MOI at 3 pfu/cell and 2 pfu/cell, respectively, in Schott flask and bioreactor. ( A ) Viable cell density pattern. ( B ) Viability pattern. The points and error bars from Schott flask assays represent the average and the standard deviation of three repetitions, respectively.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Recombinant, Standard Deviation

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Rabies protein expression profiles from coinfection performed in Schott flasks and bioreactor at BVM MO of 2.0 pfu/cell and a BVG/BVM MOI ratio of 1.5. ( A ) Kinetics profiles of rabies matrix protein (RVM) production (measured by the signal intensity of the dot blot membrane labeled with anti-RVM antibody). ( B ) Kinetics profiles of rabies G glycoprotein (RVGP) production (measured by the signal intensity of the dot blot membrane labeled with anti-RVGP antibody). ( C ) Kinetics profiles of rabies G glycoprotein (RVGP) production (measured by ELISA).

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Expressing, Dot Blot, Membrane, Labeling, Enzyme-linked Immunosorbent Assay

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Western blotting labeled with anti-RVM antibody from co-infection assays (M MOI of 2 pfu/cell, G MOI of 3 pfu/cell). ( A ) 96 h post-infection Schott flask samples. ( B ) Samples from 74 and 93 h post-infection in the bioreactor. MPM = molecular weight marker.

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Western Blot, Labeling, Infection, Molecular Weight, Marker

Journal: Vaccines

Article Title: Performance Comparison of Recombinant Baculovirus and Rabies Virus-like Particles production Using Two Culture Platforms

doi: 10.3390/vaccines11010039

Figure Lengend Snippet: Transmission electron micrographs of VLP obtained in Schott flask and bioreactor at BVM MOI of 2 pfu/cell and BVG MOI of 3 pfu/cell. ( A ) Micrograph of VLP produced in Schott flask without immunostaining. ( B ) Micrograph of VLP produced in Schott flask with immunostaining (ant- G). ( C ) Micrograph of VLP produced in benchtop bioreactor without immunostaining. ( D ) Micrograph of VLP produced in benchtop bioreactor with immunostaining (anti-G).

Article Snippet: In previous studies, these features have allowed for the demonstration of similar results in non-well-controlled culture systems, such as those provided by Schott flasks and wave bioreactors, concerning heterologous protein expression in recombinant constitutive insect cell lines [ ].

Techniques: Transmission Assay, Produced, Immunostaining